Microbiology culture media

Liquid media

Solid media

Simple media/basal media §  Nutrient broth—routine culture §  Peptone water---routine culture,basal medium for sugar fermentation test Special/Complex media(most of media other than basal media) 2 types—enriched,enrichment Enriched §  Glucose broth—blood culture Enrichment §  RCM medium—anaerobic bacteria culture §  Tetrathionate broth—salmonella §  Selenite F broth –salmonella, Shigella §  Thioglycollate broth—anaerobic bacteria §  Alkaline peptone water—vibrio

Simple-----------Nutrient agar Special Enriched media—solid media enriched with blood,serum etc. §  Blood agar §  Chocolate agar §  Loeffler’s serum slope §  L-J medium §  Dorset egg Selective media—solid media that facilitate isolation of certain bacteria §  TCBS §  DCA §  L-J §  MSA §  XLD

Defined/synthetic media-----have known quantities of all ingriedients

§  Dubo’s medium with tween 80

Differential media/indicator media

§  Wilson & blair media ---------- S.Typhi

§  Potassium tellurite in mcLeod’s medium-----------diphtheria bacilli

§  Eosin methylene blue medium

§  Mac Conkey medium

§  Mannitol salt agar

Transport media

§  Stuart’s medium

§  Alkaline peptone water

Sugar media—1% sugar  in peptone water along with appropriate indicator