Biosafety levels

CDC  Biosafety levels

Guidelines to protect microbiological workers

4 biosafety levels

		Risk group
Biosafety level-1	Agents that pose low risk to personnel & the environment Low risk of disease in healthy individuals, animals Found in water testing laboratories, high schools,colleges teaching introductory microbiology classes Standard microbiological practices are used when working Autoclaving Disinfectants Lab coats Gloves Facial protection	Agrobacterium radiobacter Aspergillus niger Bacillus thurigiensis Bacillus subtilis Canine hepatitis Varicella –chicken pox E.Coli strain K-12 Lactobacillus acidophilus Micrococcus leuteus Neurospora Pseudomonas fluorescens Serratia
Biosafety level 2	Pose moderate risk Risk  laboratory acquired infection—low Effective treatment and preventive measures are available Exposure rarely causes infection & serious disease Access to lab restricted Autoclave Gloves Face protection Lab coats	Bacteria §  MOTT §  Pneumococci §  Streptococci §  Staphylococci §  Salmonella §  Enterobacteriaciae §  E.Coli §  Clostridium sp. §  Lyme disease §  Bacillus (except—anthrax ) Viruses §  HIV §  HBV §  HCV §  HAV §  Adeno §  Calci §  Corona §  Herpes §  Mumps §  Measles §  Scrapie §  Influenza Fungi §  Cryptococcus §  Candida §  Aspergillus §  Dermatophytes
Biosafety level 3	Serious disease risk Not transmitted by casual contact Laboratory is separate /isolated building Double door entry Directional inward air-flow	Bacteria Anthrax Brucella Coxiella burnetti Tularemia MAC M.Tuberculosis Viruses Lymphocytic choriomenigitis virus Hantaan virus St. Louis encephalitis virus Japanese encephalitis  virus West nile encephalitis virus SARS corona virus Small-pox ------ variola Typhus Rift valley fever Rocky mountain spotted fever Yellow fever Malaria Trypanosomiasis Fungi Coccidiodes imitis Blastomyces dermatitides Histoplasma capsulatum Paracoccoidiodes brazileinsis
Biosafety level 4	Risk of Very serious infection Spread by casual contact No vaccines for tmt. High mortality rate	Lassa virus Marburg virus Ebola virus Herpes simiac virus H5N1 bird flu Dengue fever Crimean congo hemmorhagic fever Bolivian & argentine hemmorhagic fevers